Fig. 4: Aurora A knockdown reduces the protein stability of YAP independently of the proteasome pathway.
From: Activation of Aurora A kinase increases YAP stability via blockage of autophagy
a Aurora A depleted (shA1) and control groups (shNC) were treated with 100 μg/ml cycloheximide (CHX) for the indicated time and subjected for western blot analysis. The relative expression (right panel) are the ratios of YAP normalized to GAPDH. b Aurora A depleted (shA1) and control (shNC) A549 and c H1299 cells were treated with MG132 (10 μM) for 6 h, both Aurora A and YAP protein levels were analyzed by western blot. The relative expression shown (right panel) are mean ± S.D. of the ratios of YAP to GAPDH (n = 3, *P < 0.05; **P < 0.01). d Aurora A depleted (shA1) and control (shNC) groups were treated with CHX (100 μg/ml) and MG132 (10 μM) for 6 h and subjected for western blot analysis. The relative expression shown (right panel) are mean ± S.D. of the ratios of YAP to GAPDH (n = 3, **P < 0.01)
