Fig. 4: MicroRNA-708 (miR-708) directly targeted sphingosine kinase 2 (SPHK2) in glioma cells.

a Binding sites of miR-708 at SPHK2 3′-untranslated region (3′-UTR), SPHK2 wild-type (WT), and mutant (MT) 3′-UTR are indicated. b The luciferase activity in SPHK2 wild-type construct, instead of luciferase activity in mutant construct, was decreased when miR-708 was presented. c miR-708 decreased SPHK2 messenger RNA (mRNA) expression level in LN382 and GBM-GY cells. d miR-708 decreased SPHK2 protein levels in LN382 and GBM-GY cells. e The reversed association between miR-708 and SPHK2 expression was indicated in glioma tissues