Fig. 5: Sphingosine kinase 2 (SPHK2) was a glioma oncogene.

a SPHK2 expression was elevated in glioma tissues compared with the normal brain tissues. b 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays revealed that SPHK2 down-regulation inhibited glioma cell growth. c The colony-forming ability of glioma cells was decreased after restoration of microRNA-708 (miR-708) expression. d The number of 5-ethynyl-2′-deoxyuridine (EdU)-positive cells was decreased in the LV-miR-708 group compared with the LV-ctrl group. f Boyden assay demonstrated that miR-708 inhibited glioma cell invasion