Fig. 4: Ets-1 enhanced the activity of TGF-β1/Smad3 signaling. | Cell Death & Disease

Fig. 4: Ets-1 enhanced the activity of TGF-β1/Smad3 signaling.

From: Ets-1 deficiency alleviates nonalcoholic steatohepatitis via weakening TGF-β1 signaling-mediated hepatocyte apoptosis

Fig. 4

a, b Primary hepatocytes treated with Gfp and Ets-1 (a) or shNC and shEts-1 (b) recombinant adenovirus for 36 h. The cells were incubated with TGF-β1 (10 ng/mL) for 6 h, and the lysates were used for immunoblot analysis. (c) TGF-β1 (10 ng/mL) was added to hepatocytes for 1, 2, 4 and 6 h. The nuclear fraction was subjected to immunoblotting. d, e Primary hepatocytes were treated with siNC, siSmad3 or siEts-1 for 36 h and incubated with TGF-β1 (10 ng/mL) for 24 h. d mRNA level of Bcl2l11 is shown. e Total lysates were used for immunoblotting of Ets-1, Smad3 and Bim. Quantitative data are presented as mean ± SEM (at least three independent experiments). NS (negative significance); *P < 0.05 and **P < 0.01

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