Fig. 6: Ets-1 was elevated in the liver tissues of NASH mice.

WT mice were injected with AAV8-shNC virus and AAV8-shEts-1 virus through the tail vein for 2 weeks and divided into four groups: chow diet+shNC (n = 6), chow diet+shEts-1 (n = 6), MCD diet+shNC (n = 6), and MCD diet+shEts-1 (n = 6). The mice were fed with a chow diet or an MCD diet for 8 weeks and sacrificed. a The lysates of liver tissues were used for immunoblotting of Ets-1. b ALT and AST levels in serum, U/L: enzyme activity per liter of liquid. c Liver sections were stained with H&E, Sirius Red, and F4/80. Scale bar: 100 μm. The left panel is a representative image, and the right panel is the quantification of Sirius Red–positive and F4/80-positive areas. The results were quantified using the Image J software. d Hepatic mRNA levels of inflammation-related proteins (Tnf, Il1b and Il6) and fibrosis-related proteins (Col1A1 and Acta2) were quantified using qRT-PCR. e Liver specimens were stained with TUNEL. A representative image is shown on the left, with the corresponding statistical result on the right. f, g Total lysates (f) or nuclear lysates (g) of liver tissues were used for immunoblotting. The left panels are representative images, and the right panels are corresponding statistical results. The red arrow is referring to cleaved PARP. Quantitative data represent mean ± SEM. *P < 0.05; **P < 0.01