Fig. 5: Evaluation of necrotic cell and inflammation in the liver of CDE-fed WT and Mlkl KO mice. | Cell Death & Disease

Fig. 5: Evaluation of necrotic cell and inflammation in the liver of CDE-fed WT and Mlkl KO mice.

From: Hepatic ferroptosis plays an important role as the trigger for initiating inflammation in nonalcoholic steatohepatitis

Fig. 5

a Serum liver injury marker levels of WT and Mlkl KO mice after 18 h of the CDE diet feeding (n = 5). NS not significant. b Detection of necrotic cells in the liver of WT and Mlkl KO mice at 18 h post CDE feeding by the in vivo necrosis assay. The number of PI-positive cells was evaluated in 10 non-overlapping fields of view for each biological sample. The data are shown as the means ± SEM (n = 5). NS not significant. c Gene expression analysis of inflammatory cytokines by quantitative RT-PCR. All data are normalized to Gapdh and shown as the means ± SEM (n = 5; *P < 0.05)

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