Fig. 7: Evaluation of the effect of iron chelators in the CDE diet model. | Cell Death & Disease

Fig. 7: Evaluation of the effect of iron chelators in the CDE diet model.

From: Hepatic ferroptosis plays an important role as the trigger for initiating inflammation in nonalcoholic steatohepatitis

Fig. 7

a Serum liver injury marker levels in the CDE-fed mice treated with vehicle or DFO (100 mg/kg) (n = 9). NS not significant. The serum data are shown as dot plots and mean. b Serum liver injury marker levels of the CDE-fed mice treated with vehicle or DFP (100 mg/kg) (n = 9; **P < 0.01, ***P < 0.001). c Detection of necrotic cells in the CDE-fed mice treated with vehicle or DFP. The number of PI-positive cells was evaluated in 10 non-overlapping fields of view for each biological sample. The data are shown as the means ± SEM (n = 9; **P < 0.01). d Immunohistochemical analysis of CDE-fed mouse treated with vehicle or DFP for CD11b. The number of CD11b-positive cells was evaluated in 10 non-overlapping fields of view for each biological sample. The data are shown as the mean ± SEM (n = 9; *P < 0.05). e Gene expression analysis of inflammatory cytokines by quantitative RT-PCR. The gene expression data are normalized to Gapdh and shown as the means ± SEM (n = 9; **P < 0.01, ****P < 0.0001). Scale bar = 100 µm

Back to article page