Fig. 5: IRF-1 suppresses MIR17HG promoter activity.

a Two putative IRF-1 transcriptional binding sites in the MIR17HG gene promoter region. b ChIP assays of MKN45 and SGC7901 cells were performed to characterise the recruitment of IRF-1 to the MIR17HG gene promoter. c MKN45/Lv-IRF-1 and SGC7901/Lv-IRF-1 cells were treated with 4 μg/ml Dox for 24 hours, and ChIP analyses were performed to assess the binding of IRF-1 to the MIR17HG gene promoter. d Schematic diagram of the reporter constructs of the wild-type (WT) and mutant (MUT) MIR17HG promoter binding site-1 fragment. e The indicated MIR17HG reporter constructs were co-transfected into MKN45 and SGC7901 cells with IRF-1 plasmids (TR-IRF-1) or normal control plasmids (TR-NC), and the luciferase activities were then measured and analysed. Three independent experiments were performed in triplicate (N = 3). *P < 0.05, as determined by paired Student’s t test. The data b, c and e are presented as the means ± SDs