Fig. 2: FOXP4-AS1 facilitated PCa cell growth in vitro.

a The CCK-8 assay was used to detect cell viability after overexpression or knockdown of FOXP4-AS1. b, c Proliferative ability of LNCaP cell transfected with FOXP4-AS1 expression vector or PC-3 cell transfected with sh-FOXP4-AS1 was assessed by the colony formation assay and EdU assay. Scale bar for EdU staining: 100 μm. d Membrane potential in FOXP4-AS1-overexpressed or -downregulated PCa cells was measured by JC-1 staining. Scale bar = 200 μm. e Caspase-3 activity was tested in two PCa cells that were separately transfected with FOXP4-AS1 expression vector or sh-FOXP4-AS1#1. *P < 0.05, **P < 0.01