Fig. 5: FOXP4-AS1 acted as the molecular sponge of miR-3184-5p. | Cell Death & Disease

Fig. 5: FOXP4-AS1 acted as the molecular sponge of miR-3184-5p.

From: LncRNA FOXP4-AS1 is activated by PAX5 and promotes the growth of prostate cancer by sequestering miR-3184-5p to upregulate FOXP4

Fig. 5

a, b FOXP4-AS1 was predominantly located in the cytoplasm of PCa cells. Scale bar = 200 μm. c Ago 2-RIP revealed that both FOXP4-AS1 and FOXP4 were enriched in Ago 2 containing beads. d Five miRNAs that had complementary base paring with both FOXP4-AS1 and FOXP4 were predicted from starBase and DIANA. e Enrichment of five miRNAs in MS2 with or without FOXP4-AS1. f miR-3184-5p and miR-423-5p were detected in cells transfected with FOXP4-AS1 expression vector or sh-FOXP4-AS1#1. g The predicted binding sequence between FOXP4-AS1 and miR-3184-5p. h The luciferase reporter assay was conducted in cells transfected with miR-3184-5p mimics or miR-NC to examine the luciferase activity of FOXP4-AS1-WT and FOXP4-AS1-MUT. *P < 0.05, **P < 0.01

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