Fig. 8: PAX5 transcriptionally activated FOXP4-AS1 and FOXP4 in PCa cells. | Cell Death & Disease

Fig. 8: PAX5 transcriptionally activated FOXP4-AS1 and FOXP4 in PCa cells.

From: LncRNA FOXP4-AS1 is activated by PAX5 and promotes the growth of prostate cancer by sequestering miR-3184-5p to upregulate FOXP4

Fig. 8

a PAX5 expression in paired PCa samples and normal samples. b Positive expression correlation between PAX5 and FOXP4-AS1 or FOXP4 in PCa tissues. c DNA motif of PAX5 and predicted five putative binding sites of PAX5 in FOXP4-AS1 or FOXP4 promoter. d The luciferase reporter assay demonstrated that site 1 of FOXP4-AS1 promoter (from −1475 to −1493) and site 5 of FOXP4 promoter (from −409 to −427) were potentially responsible for the binding of PAX5 to FOXP4-AS1 or FOXP4 promoter. e The ChIP assay showed that there was a strong affinity of PAX5 to FOXP4-AS1 or FOXP4 promoter. f Luciferase activity analysis revealed that the luciferase activity of reporter vector containing FOXP4-AS1 promoter or FOXP4 promoter was not changed by PAX5 after mutations in site 1 or site 5. *P < 0.05, **P < 0.01

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