Fig. 4: EPHA7 was transcriptionally silenced by EZH2 through elevating the methylation on EPHA7 promoter. | Cell Death & Disease

Fig. 4: EPHA7 was transcriptionally silenced by EZH2 through elevating the methylation on EPHA7 promoter.

From: Long noncoding RNA SNHG14 facilitates colorectal cancer metastasis through targeting EZH2-regulated EPHA7

Fig. 4

a UCSC indicated that EZH2 was one of the transcription factors of EPHA7. b The expression of EZH2 in COAD and READ tissues and the correlation between the expression of EPHA7 and EZH2 in COAD were predicted by TCGA database. c Relative expression of EZH2 in CRC cell lines and NCM460 cells detected by qRT-PCR. d, e The expression of EZH2 and EPHA7 in EZH2-inhibited LoVo cells or EZH2-overexpressed HT-29 cells was assayed with qRT-PCR. f The binding of EZH2 to EPHA7 promoter was validated via conducting ChIP assay. g, h The influence of EZH2 on EPHA7 transcription was evaluated by luciferase reporter assay and ChIP assay. *P < 0.05, **P < 0.01

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