Fig. 6: Apoptosis induced by LZ-101 was abolished after inhibition of autophagosome formation in A549 cells. | Cell Death & Disease

Fig. 6: Apoptosis induced by LZ-101 was abolished after inhibition of autophagosome formation in A549 cells.

From: LZ-101, a novel derivative of danofloxacin, induces mitochondrial apoptosis by stabilizing FOXO3a via blocking autophagy flux in NSCLC cells

Fig. 6

a FOXO3a was detected by western blot in the presence of 3-MA (4 mM) in A549 cells treated 15 μM LZ-101. b Annexin-V/PI staining assay by flow cytometry in the presence of 3-MA (4 mM) in A549 cells treated with 15 μM LZ-101. c ATG5 and ATG7 were detected by western blot after transfection of ATG5 or ATG7 siRNA into A549 cells. d Annexin-V/PI staining assay by flow cytometry in A549 cells treated with 15 μM LZ-101 and transfected ATG5 or ATG7 siRNA. Data are presented as mean ± SD. *P < 0.05, **P < 0.01 compared with DMSO group

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