Fig. 8: Effect of rhHMGB1 on WT and TLR9KO mice post-MI angiogenesis. | Cell Death & Disease

Fig. 8: Effect of rhHMGB1 on WT and TLR9KO mice post-MI angiogenesis.

From: TLR9 is essential for HMGB1-mediated post-myocardial infarction tissue repair through affecting apoptosis, cardiac healing, and angiogenesis

Fig. 8

a HIF-1α, VEGFA, and CD31 expression in the heart sections of the WT and TLR9KO mice post-MI after 14 days determined by immunohistochemistry (bar = 50 μm). b, c Representative western blots and quantification of HIF-1α, VEGFA, CD31, and PCNA in the cardiac tissues from the WT and TLR9KO mice post-MI after 14 days (n = 6). d, e Immunohistochemical staining with anti-CD31 and anti-α-SMA antibody in the border zone of the WT and TLR9KO mice heart at 14 days post-MI, capillary densities (CD31-positive cells/HPF) and arteriole densities (α-SMA-positive cells/LPF) were acquired (n = 6, bar = 50 μm). f Nuclear extracts were prepared at WT and TLR9KO mice hearts and analyzed by western blot for the nuclear accumulation of RelA and HIF-1a (n = 6). NS indicates not significant; *P < 0.05; §P < 0.05 vs. WT MI group

Back to article page