Fig. 1: AR expression in breast cancer cell-lines.

a Blot shows the expression of AR protein. Experimental protocol is as described in the “Materials and methods”. Blots were also probed for β-actin (bottom) to verify equal amounts of protein loaded in each lane. The ER(+) cells (MCF-7 and ZR-75-1) are expressing the AR. The ER(−) MDA-MB-231 almost have no AR. b The densitometric evaluations are calculated by ImageJ. These data are representative of three separate experiments, each in triplicate; bars, SEM