Fig. 6: Effects of blocking of the AR or regulation of its cellular expression on the ability of compounds to influence the proliferation of breast cancer cells. | Cell Death & Disease

Fig. 6: Effects of blocking of the AR or regulation of its cellular expression on the ability of compounds to influence the proliferation of breast cancer cells.

From: The beneficial androgenic action of steroidal aromatase inactivators in estrogen-dependent breast cancer after failure of nonsteroidal drugs

Fig. 6

Cells were cultured and treated as described in the legend of Fig. 2. Cell growth was measured as described in “Materials and methods”. a and b Opposing effect of hydroxyflutamide (100 nM) given simultaneously to the inhibitory effect of test compounds (100 nM) on the growth of MCF-7 and ZR-75-1 cells. c and d The influence of changes in expression of the AR in MCF-7 cells (downregulation) and MDA MB-231 cells (upregulation) on the proliferative response to the tested compounds (100 nM). In MCF-7 cells inhibition of proliferation is mitigated, but in MDA-MB-231 cells proliferation is reduced. The blot shows the expression of the AR protein. Cell growth is expressed as the ratio of the number of cells in the experimental well to that in control wells (untreated cells). The columns represent the mean of three experiments, each with five replicates; bars, SEM. *P ≤ 0.05, **P ≤ 0.01 vs. control

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