Fig. 4: Effect of IL-32γ on the expression of cell-cycle and apoptosis-related proteins in cancer stem cells.
From: IL-32γ suppresses lung cancer stem cell growth via inhibition of ITGAV-mediated STAT5 pathway
a CD133+ A549 cells were transfected with human IL-32γ, after 24 h of post transfection; cell survival and apoptosis regulatory proteins, including PCNA, Survivin, BID, tBID, PUMA, Bcl-2, and caspase-3 were detected using specific antibodies. b Cell-cycle regulatory proteins, including CDK1, CDK2, CDK4, CDK6, and Cyclin B and D1 were detected using specific antibodies. c Expression of MAPK pathway related proteins, including pERK, pJNK, pp38, and pAKT was detected using specific antibodies. d Activation of STAT pathway regulatory proteins was detected using specific antibodies, such as pSTAT1, pSTAT3, and pSTAT5. β-actin protein was used as an internal control. Each band is representative of three independent experiments
