Fig. 1: Separation and identification of chondrocytes-derived exosome-like vesicles. | Cell Death & Disease

Fig. 1: Separation and identification of chondrocytes-derived exosome-like vesicles.

From: The exosome-like vesicles from osteoarthritic chondrocyte enhanced mature IL-1β production of macrophages and aggravated synovitis in osteoarthritis

Fig. 1

a The nondegraded cartilage explants or the degraded cartilage explants from donors were cultured in serum-free cell culture media. Then the supernatant extracellular vesicles obtained by ultrafiltration assay (10 KDa MWCO) were collected and subjected to NanoSight analysis. b The corresponding statistical graph for the size distribution of extracellular vesicles from different samples were shown (n = 3), ANOVA with Bonferroni’s multiple comparison test was used, ***p< 0.001. c Human primary chondrocytes from OA patients were isolated and cultured in serum-free cell culture media. Then the supernatant exosome-like vesicles extracted using ultracentrifugation assay (left) and ultrafiltration assay (right) were separately subjected to NanoSight detection. d The morphology of exosome-like vesicles from human primary chondrocytes were observed under transmission electron microscope, and the representative image was shown. e The indicated protein level in exosome-like vesicles from human primary chondrocytes were detected by Western blot. f The size distribution of Dio-labeled exosome-like vesicles from human primary chondrocytes were measured by ImageStreamX using 100 nm FITC-nanoparticles as standard control

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