Fig. 3: Autophagy inhibition contributes to ChCILpre-exos-mediated production of mature IL-1β. | Cell Death & Disease

Fig. 3: Autophagy inhibition contributes to ChCILpre-exos-mediated production of mature IL-1β.

From: The exosome-like vesicles from osteoarthritic chondrocyte enhanced mature IL-1β production of macrophages and aggravated synovitis in osteoarthritis

Fig. 3

a PMA-induced THP-1 cells were treated with pChCNCpre-exos or pChCILpre-exos in the presence of LPS and then the indicated protein levels were detected by western blot assay. b PMA-induced THP-1 cells were treated with swChCNCpre-exos or swChCILpre-exos in the presence of LPS for 24 h, combined with 100 nM Baf A1 for aftermost 3 h. Subsequently, the indicated protein levels were detected by Western blot assay. swChCILpre-exos, exosome-like vesicles from IL-1β-pretreated SW1353 cells; swChCNCpre-exos, exosome-like vesicles from ddH2O-pretreated SW1353 cells. c PMA-induced THP-1 cells were given with the indicated treatments and then immunofluorescence was used to detect the expression pattern of LC3 (Green). DAPI staining was taken to mark the nucleus. The corresponding statistical graph for the average number of LC3 puncta per cell was shown (d). e PMA-induced THP-1 cells were transfected with ATG7 siRNA and the control siRNA and then given with the indicated treatments. Subsequently, the supernatant IL-1β was measured by ELISA assay. ANOVA with Bonferroni’s multiple comparison test was used, **p < 0.01; *p < 0.05; ns, no significance

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