Fig. 7: The miR-449a-5p inhibitor partially reverse the ChCILpre-exos-mediated autophagy inhibition and production of mature IL-1β. | Cell Death & Disease

Fig. 7: The miR-449a-5p inhibitor partially reverse the ChCILpre-exos-mediated autophagy inhibition and production of mature IL-1β.

From: The exosome-like vesicles from osteoarthritic chondrocyte enhanced mature IL-1β production of macrophages and aggravated synovitis in osteoarthritis

Fig. 7

a PMA-induced THP-1 cells were transfected with the inhibitor-miR-449a-5p and the negative control, followed with pChCNCpre-exos or pChCILpre-exos in the presence of LPS. Then the LC3I/II protein level was detected by Western blot. b, c PMA-induced THP-1 cells were treated as a and then the immunofluorescence assay was taken to measure the LC3-puncta number per cell (b) and the ratio of ASC-specking cells (c). d PMA-induced THP-1 cells were treated as a and then the supernatant IL-1β was measured using ELISA assay. ANOVA with Bonferroni’s multiple comparison test was used, *p < 0.05; ns, no significance

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