Fig. 5: Overexpression of IFITM3 promotes tumor cell metastasis. | Cell Death & Disease

Fig. 5: Overexpression of IFITM3 promotes tumor cell metastasis.

From: IFITM3 promotes bone metastasis of prostate cancer cells by mediating activation of the TGF-β signaling pathway

Fig. 5

a, b The overexpressing efficiency of IFITM3 assessed by qPCR and Western blot, and mTOR/p-mTOR, P38/P-P38 proteins were also analyzed by Western blot in DU145 cells. c MTT shows no significant difference in DU145 cell proliferation in all groups. d Transwell migration assay demonstrated that the number of cells migrating through the 8 μm diameter pores in both oeIFITM3(#1) and oeIFITM3(#2) groups was increased significantly as compared with the control group. e, f DU145 cell counts and the absorbance at 570 nm of Lv-oeIFITM3 group were significantly higher than those in the control group. g The expression of snail, MMPs, and fibronectin in EMT pathway changed after IFITM3 overexpression. h Changes in metastasis-related molecules after overexpressing IFITM3 by qPCR analysis

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