Fig. 6: IFITM3 affects PCa bone metastasis through regulation of The TGF-β-induced Smads signaling pathway. | Cell Death & Disease

Fig. 6: IFITM3 affects PCa bone metastasis through regulation of The TGF-β-induced Smads signaling pathway.

From: IFITM3 promotes bone metastasis of prostate cancer cells by mediating activation of the TGF-β signaling pathway

Fig. 6

a Western blot analysis of an anti-HA or anti-FLAG coimmunoprecipitation (IP) experiment performed in cell extracts containing transiently expressed FLAG-IFITM3 and Smad4-HA. b The lentivirus was successfully transfected and the result was measured in fluorescence (magnification × 100). c, d Changes in ERK and Smad2 phosphorylation levels after IFITM3 knockdown or overexpression as shown by Western blot analysis. 10 μM SB431542 significantly reversed the enhancing expression of p-Smad2 and p-ERK induced by TGF-β (5 ng/mL) in IFITM3 overexpressed DU145 cells. The coordination of TGF-β and SB431542 is shown. e, f SB431542 (10 μM) significantly reversed the mRNA expression of FGF2 and PTHrP induced by TGF-β (5 ng/mL) in oeIFITM3 groups

Back to article page