Fig. 1: Serum deprivation and oxidative injury inhibited the expression and secretion of CTRP3 in MSCs. | Cell Death & Disease

Fig. 1: Serum deprivation and oxidative injury inhibited the expression and secretion of CTRP3 in MSCs.

From: C1q/tumor necrosis factor-related protein-3-engineered mesenchymal stromal cells attenuate cardiac impairment in mice with myocardial infarction

Fig. 1

a, b Surface marker molecules expressed in passage 4 MSCs were analyzed by flow cytometry and quantified by image analysis of positive cells. Data are means ± SEMs (n = 4). c Representative image of MSCs morphology (passage 4) and multilineage differentiation capacity into osteoblasts and adipocytes, Images were at original magnification, ×100. d RT-PCR was used to evaluate CTRP3 mRNA levels in MSCs exposed to serum deprivation and hypoxia (n = 4). e, f Western blotting and quantification of CTRP3 in MSCs (n = 6). g RT-PCR analysis for MSCs treated with serum deprivation plus 400 μM H2O2 for 24 h (n = 4). h Western blotting and quantification of CTRP3 in MSCs cell lysates (n = 6). i Western blots and quantification of CTRP3 in conditioned medium (CM) from MSCs treated with serum deprivation plus H2O2 (n = 5). Data are means ± SEMs. *P < 0.05; **P < 0.01; ***P < 0.001 versus the control

Back to article page