Fig. 5: Transplanted hADSC-MNs exert suppressive effect on the inflammatory response in SCI mouse models. | Cell Death & Disease

Fig. 5: Transplanted hADSC-MNs exert suppressive effect on the inflammatory response in SCI mouse models.

From: Differentiation of human adipose-derived stem cells into neuron/motoneuron-like cells for cell replacement therapy of spinal cord injury

Fig. 5

a In the injury site, striking glial activation was present in the PBS control group as revealed by high expression of GFAP. Scale bar: 250 and 50 μm (for A 1–3). b In the hADSC-MN transplanted group, GFAP expression was significantly attenuated by the presence of GFP-positive transplanted cells, demonstrating their anti-inflammatory effects. Scale bar: 250 and 50 μm (for B 1–3). c–e Immunocytokine secretion profiling in both spinal cord lysates and blood serum is analyzed in various groups; c the cytokine profiling in the spinal cord lysates; d the cytokine profiling in the blood serum; SCI mice injected with PBS or hADSC-MN 7 days after surgery and collected spinal cord lysate supernatant 7 days after injection was grouped as SCI-7D-7D-PBS and SCI-7D-7D-hADSC-MN, respectively, and the scramble group labeled as SCI-Sham. The experimental protocol strictly follows the MCYTOMAG-70K-PX32 (Merk Millipore, Lot#2618731); e the variation of cytokine profiling correlated with the corresponding macrophage and pro- or anti-inflammation factors changes; Scale bar: 100 μm

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