Fig. 1: HnRNP L is found to be important for CSR recombination in CH12F3-2A cells and DNA-break level in CRC cells. | Cell Death & Disease

Fig. 1: HnRNP L is found to be important for CSR recombination in CH12F3-2A cells and DNA-break level in CRC cells.

From: Heterogeneous nuclear ribonucleoprotein L facilitates recruitment of 53BP1 and BRCA1 at the DNA break sites induced by oxaliplatin in colorectal cancer

Fig. 1

a FACS profiles of IgA switching in CH12F3-2A cells transfected with hnRNP L and control siRNAs. Data are representative of three independent experiments, as shown in (b). The error bars represent the SEM; (+) and (−) represent the present and absence of CIT stimulation. SEM values were derived from three independent experiments. ***P < 0.001, unpaired t test. c Western blot analysis showing the knockdown efficiency of hnRNP L and AID. d DSBs determination by γH2AX ChIP assay using hnRNP L and control siRNAs in CH12F3-2A cells. The presence or absence of CIT stimulation is indicated by (+) or (−), respectively. SEM values were derived from three independent experiments. *P < 0.05, ns: no significant difference, unpaired t test. e Representative images of γH2AX staining in different human colorectal cancer cell lines treated with control hnRNP L siRNA. Nuclei were stained with DAPI. Scale bar represents 10 μm. f Histograms show the numbers of γH2AX foci per nucleus. Approximately 35–45 nuclei were evaluated for γ-H2AX foci formation for each sample, data are presented as mean ± s.e.m. *P < 0.05, **P < 0.01, ***P < 0.001, unpaired t test. g Western blot analysis showing the knockdown efficiency of hnRNP L in the indicated colorectal cancer cell lines

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