Fig. 3: Absence of hnRNP L enhances the sensitivity of oxaliplatin in colorectal cancer cells. | Cell Death & Disease

Fig. 3: Absence of hnRNP L enhances the sensitivity of oxaliplatin in colorectal cancer cells.

From: Heterogeneous nuclear ribonucleoprotein L facilitates recruitment of 53BP1 and BRCA1 at the DNA break sites induced by oxaliplatin in colorectal cancer

Fig. 3

a CCK-8 assay was used to assess cell viability in colorectal cancer cells after treatment by increasing concentrations of oxaliplatin at 48 h with siControl or sihnRNP L siRNA. IC50 values of different colorectal cancer cell lines treated with siControl or sihnRNP L under oxaliplatin condition. IC50 of siControl vs. sihnRNP L, SW480 cells, 2.69 ± 0.15 vs. 1.07 ± 0.26, **P < 0.01; SW620 cells, 3.98 ± 0.29 vs. 0.50 ± 0.19, ***P < 0.001; HT29, 13.38 ± 2.1 vs. 10.25 ± 1.9, *P < 0.05; HCT116, 4.69 ± 0.84 vs. 1.52 ± 0.91, **P < 0.01. b Analysis of apoptosis by Annexin-V/PI double staining. Cells treated with sihnRNP L, cells treated with 15 µM oxaliplatin, and cells treated with both sihnRNP L and 15 µM oxaliplatin. Apoptosis data are shown as mean of quartic experiments. Statistical significance was evaluated by unpaired t test. **P < 0.01, ***P < 0.001, ns: no significant difference

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