Fig. 4: Deficiency of hnRNP L increases double-strand DNA breaks in response to treatment with oxaliplatin. | Cell Death & Disease

Fig. 4: Deficiency of hnRNP L increases double-strand DNA breaks in response to treatment with oxaliplatin.

From: Heterogeneous nuclear ribonucleoprotein L facilitates recruitment of 53BP1 and BRCA1 at the DNA break sites induced by oxaliplatin in colorectal cancer

Fig. 4

a Representative images of γH2AX staining in different human colorectal cancer cell lines treated as indicated. The nuclei were stained with DAPI. Scale bar represents 10 μm. b Scatter dot plots show the numbers of γH2AX foci per nucleus. Approximately 35–45 nuclei were evaluated for γ-H2AX foci formation for each sample, data are presented as mean ± s.e.m. ***P < 0.001, unpaired t test. c Western blot analysis showing the expression and phosphorylation levels of critical DNA repair factors in the indicated colorectal cancer cell lines treated with sihnRNP L and/or oxaliplatin. d Quantitative analysis of the protein levels detected by western blotting. Data are presented as mean ± s.e.m. of three experiments. *P < 0.05, **P < 0.01, ***P < 0.001, ns: no significant difference, compared to siControl transfected cells incubated with DMSO, unpaired t test

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