Fig. 5: HnRNP L is critical for recruitment of 53BP1 and BRCA1 at the DNA break sites induced by oxaliplatin. | Cell Death & Disease

Fig. 5: HnRNP L is critical for recruitment of 53BP1 and BRCA1 at the DNA break sites induced by oxaliplatin.

From: Heterogeneous nuclear ribonucleoprotein L facilitates recruitment of 53BP1 and BRCA1 at the DNA break sites induced by oxaliplatin in colorectal cancer

Fig. 5

a Representation of the siRNA-resistant hnRNP L mutants used in DNA repair factors foci complementation experiments. b Expression of exogenous hnRNP L-FLAG was confirmed by western blotting. c SW620 cells were treated as indicated and stained for γ-H2AX, 53BP1, ATM, and BRCA1 in order to reveal the recruitment of these DNA repair factors to the DNA break sites. Scale bar represents 10 μm. d Scatter dot plots show the numbers of γ-H2AX, 53BP1, ATM, and BRCA1 foci per nucleus. Approximately 35–45 nuclei were evaluated for foci formation for each sample, data are presented as mean ± s.e.m. **P < 0.01, ***P < 0.001, unpaired t test

Back to article page