Fig. 6: HnRNP L inhibits both HR and NHEJ at I-Scel-induced DNA breaks. | Cell Death & Disease

Fig. 6: HnRNP L inhibits both HR and NHEJ at I-Scel-induced DNA breaks.

From: Heterogeneous nuclear ribonucleoprotein L facilitates recruitment of 53BP1 and BRCA1 at the DNA break sites induced by oxaliplatin in colorectal cancer

Fig. 6

a Representative flow cytometry for the DSB repair reporter. Ten thousand cells per sample were analyzed. b Schematics of the DRR consists of a promoter and resistance cassette fused to a T2A peptide and two inverted ISce1 sites, followed by GFP. An exogenous donor containing mCherry is conceived for HR. Repair by NHEJ or HR leads to GFP or mCherry expression, respectively. c Percentages of GFP + and mCherry + cells, gated on BFP +. Data are presented as mean ± s.e.m. of of five replicates, statistical significance was evaluated by unpaired t test. *P < 0.05, **P < 0.01, ***P < 0.001

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