Fig. 2: LC3 and Beclin 1 expression, and ultrastructure of autophagy in Hcy-treated NSCs exposed to OGD/R.
From: Homocysteine enhances neural stem cell autophagy in in vivo and in vitro model of ischemic stroke
a Representative western blot for LC3. Quantification of LC3 II/LC3 I graphed below. b Representative western blot for Beclin 1. Bar graphs show semiquantitative levels of Beclin 1 as determined by band density analysis. Data from three independent experiments. Note that the quantification of Beclin 1 or LC3 II/LC3 I ratio of other groups is normalized to the normal group. Data are expressed as mean ± SD. a P < 0.05 compared with the normal group. b P < 0.05 compared with the OGD/R group. c Representative electron photomicrographs of autophagosomes. Three panels in the bottom show the magnified images of the insets. Normal morphology of cytoplasm was indicated by the asterisk symbol. The autophagy formation was indicated by the hash symbol in the OGD/R group. The arrowheads show characteristic autophagosomes in the OGD/R + HCY group. Scale bar = 2 μm
