Fig. 3: Hcy increases LC3 expression in the NSCs of SVZ after brain ischemia-reperfusion injury.
From: Homocysteine enhances neural stem cell autophagy in in vivo and in vitro model of ischemic stroke
a Representative images of double immunofluorescence staining for LC3 (green) and Nestin (red) in the SVZ for all experimental groups at 3 days (left panel) and 7 days (right penal) after operation, respectively. Scale bar = 50 μm. b Double immunofluorescent staining revealed accumulation of LC3 punctuated foci per cell. Green color, LC3 staining; red, Nestin staining; yellow, co-localization of LC3 and Nestin. Autophagosomes are recognized as punctate structures (white arrow). Scale bar = 10 μm. c The number of LC3 puncta mean ± SD per cell was counted and presented graphically. Data from three independent experiments. a P < 0.05 compared with the SHAM group. b, d P < 0.05 compared with the MCAO group. c P < 0.05 compared with the MCAO + HCY group
