Fig. 1: TOPK Inhibits autophagy in glioma cells.
From: TOPK inhibits autophagy by phosphorylating ULK1 and promotes glioma resistance to TMZ
a The expression of TOPK, LC3-II, and P62 in clinical specimens. P62, LC3B, TOPK, and Tubulin were analyzed using western blots. b The expression of TOPK, LC3-II, and P62 in four glioma cells. P62, LC3B, TOPK, and Tubulin were analyzed using western blots. c Differing concentrations of OTS964 were used to treat Hs683 cells for 24 and 48 h, respectively. Cytotoxicity was measured by MTT assay (right). Hs683 cells were treated with different concentrations of OTS964 for 48 h then the whole cell lysates were analyzed by western blots using indicated antibodies (left). d Differing concentrations of OTS964 were used to treat H4 cells for 24 h and 48 h, respectively. Cytotoxicity was measured by MTT assay (right). H4 cells were treated with different concentrations of OTS964 for 48 h, then the whole cell lysates were analyzed by western blots using indicated antibodies (left). e TOPK knockdown in Hs683 (left) and H4 (right) cells enhanced the expression of LC3-II and decreased the expression of P62. TOPK-silencing-Hs683 and TOPK-silencing-H4 cells were harvested at 80% confluence, and resolved by SDS–PAGE and analyzed using western blots. Data represent the results of triplicate experiments. f TOPK overexpression in U118MG reduced the expression of LC3-II and increased the expression of P62. pcDNA3 and pcDNA3-HA-TOPK were stably transfected into U118MG cells as indicated. P62, LC3B, HA-TOPK, and Tubulin were analyzed using western blots
