Fig. 2
From: Inhibition of transmembrane TNF-α shedding by a specific antibody protects against septic shock
tmTNF-α Ab decreases LPS-induced production of proinflammatory cytokines. THP-1-derived macrophages (a, e, i), primary human monocytes (b, f, j), Raw264.7 (c, g, k), and murine peritoneal macrophages (d, h, l) were stimulated with 100 ng/ml LPS, combined with 2 μg/ml human tmTNF-α mAb or murine tmTNF-α pAb. The same amount of isotype antibody IgG or normal serum IgG served as a control. The stimulation time was 4 h for detection of mRNA levels by real-time PCR and 10 h for detection of cytokine concentrations by ELISA. The production of IL-1β (a–d), IL-6 (e–h), and IL-10 (i–l) was detected at mRNA (upper panels) and protein (lower panels) levels. All data are presented as means ± SEM of at least three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001
