Fig. 1: CSRP3 knockout does not affect pluripotency of hESCs and differentiation into hESC-CMs. | Cell Death & Disease

Fig. 1: CSRP3 knockout does not affect pluripotency of hESCs and differentiation into hESC-CMs.

From: MLP-deficient human pluripotent stem cell derived cardiomyocytes develop hypertrophic cardiomyopathy and heart failure phenotypes due to abnormal calcium handling

Fig. 1

a Structure of the CSRP3 locus showing localization of gRNA for epiCRISPR/Cas9 editing. b Sequence chromatograms demonstrate one base insertion in CSRP3 knockout (MLP KO) colonies. c Immunofluorescence staining of MLP KO colonies for the pluripotency markers TRA-1-81 and OCT4. Scale bar, 50 μm. d Immunoblot analysis of MLP in WT and MLP KO CMs at day 15. e Protocol for cardiac differentiation using small molecule-based methods. f, g Flow cytometry analysis for TNNT2 from representative WT and MLP KO differentiation protocols prior to purification at day 10. h Immunostaining for expression of MYL2 (green) and MYL7 (red) in WT and MLP KO CMS at day 30. i Quantification of ventricular marker MYL2 in WT (n = 382) and MLP KO CMS (n = 403). Scale bar, 50 μm. Results are presented as means ± S.E.M. of three independent experiments. ns, not significant, unpaired two-sided Student’s t test

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