Fig. 6: Exacerbation of HF in response to β-adrenergic stimulation in MLP-deficient hESC-CMs. | Cell Death & Disease

Fig. 6: Exacerbation of HF in response to β-adrenergic stimulation in MLP-deficient hESC-CMs.

From: MLP-deficient human pluripotent stem cell derived cardiomyocytes develop hypertrophic cardiomyopathy and heart failure phenotypes due to abnormal calcium handling

Fig. 6

a, b Representative line-scan images from WT and MLP KO hESC-CMs after 1 week of 10 μM ISO treatment beginning at day 15 of cardiac differentiation. c–e Quantification of peak, time to peak, and calcium transient duration in WT, MLP KO, WT/ISO, and MLP KO/ISO hESC-CMs (WT n = 13, WT/ISO n = 15, MLP KO n = 13, and MLP KO/ISO n = 12). e Heatmap representations of hypertrophy related gene expression in WT, MLP KO, WT/ISO, and MLP KO/ISO hESC-CMs (n = 3). Results are presented as means ± S.E.M. of three independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001; ns, not significant, unpaired two-sided Student’s t test

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