Fig. 3: SIRT3 deficiency enhances the suppression of BAT mitochondrial biogenesis. | Cell Death & Disease

Fig. 3: SIRT3 deficiency enhances the suppression of BAT mitochondrial biogenesis.

From: Sirtuin 3-mediated pyruvate dehydrogenase activity determines brown adipocytes phenotype under high-salt conditions

Fig. 3

a Electron micrograph of mitochondria from BAT of WT and SIRT3-KO mice (magnification, ×10,000 and ×20,000). b, c Morphometric analysis of mitochondrial surface area and density. d BAT sections were prepared from WT and SIRT3-KO mice and labelled with an antibody against UCP-1 (red); nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI; blue) (n = 3/group). Scale bar: 50 μm. e BAT sections were labelled with an antibody against TOM-20 (red); nuclei were stained with DAPI (blue) (n = 3/group). Scale bar: 50 μm. f Representative western blot and g quantitative analyses of UCP-1 and TOM-20. h Representative western blot and i quantitative analyses of PGC-1α and complex I levels, shown as fold increase relative to the WT NS group. Data represent as mean ± SEM (n = 10/group). *p < 0.05 vs. genotype-matched NS mice; #p < 0.05 vs. WT HS mice

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