Fig. 4: SIRT3 deficiency accelerates the conversion of BAT to WAT. | Cell Death & Disease

Fig. 4: SIRT3 deficiency accelerates the conversion of BAT to WAT.

From: Sirtuin 3-mediated pyruvate dehydrogenase activity determines brown adipocytes phenotype under high-salt conditions

Fig. 4

a Representative western blot and b semi-quantitative analysis of SIRT3 level in brown adipocytes from WT and SIRT3-KO mice. c Brown adipocytes from WT and SIRT3-KO mice were stained with BODIPY (green) and MitoTracker Red (red). Scale bar: 50 μm. d Representative western blot and e semi-quantitative analysis of perilipin-1 and Cidea levels in brown adipocytes. f Representative western blot and g semi-quantitative analysis of UCP-1 and complex I levels in brown adipocytes. h D-loop to 18S rDNA ratio. i Representative western blot and j semi-quantitative analysis of PGC-1α level. k mRNA abundance of Nrf1 and TFAM. Results are shown as fold increase relative to the WT control and represent mean ± SEM of four independent experiments. *p < 0.05 vs. WT control; #p < 0.05, ##p < 0.05 vs. WT NaCl

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