Fig. 5: Restoring SIRT3 expression prevents high salt-induced BAT conversion to WAT. | Cell Death & Disease

Fig. 5: Restoring SIRT3 expression prevents high salt-induced BAT conversion to WAT.

From: Sirtuin 3-mediated pyruvate dehydrogenase activity determines brown adipocytes phenotype under high-salt conditions

Fig. 5

a Representative western blot and b quantitative analysis of SIRT3 in WT, SIRT3-KO and SIRT3-reconstruction brown adipocytes treated with or without NaCl. c The oxygen consumption rate (OCR) of brown adipocytes measured with XFe96 Extracellular Flux Analyzer. d Statistical analysis of OCR. e Representative western blots and f semi-quantitative analyses of PGC-1α, UCP-1 and complex I; and g, h perilipin-1 and Cidea levels in WT, SIRT3-KO and SIRT3-reconstruction brown adipocytes treated with or without NaCl. Results are shown as fold increase relative to the WT control and are presented as mean ± SEM of four independent experiments. *P < 0.05, **P < 0.01 vs. WT control; #P < 0.05, ##P < 0.01 vs. WT-NaCl. $P < 0.05, $$P < 0.01 vs. SIRT3-KO-NaCl

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