Fig. 6: Acetylation of PDHA1 K83 alters mitochondrial function of brown adipocytes. | Cell Death & Disease

Fig. 6: Acetylation of PDHA1 K83 alters mitochondrial function of brown adipocytes.

From: Sirtuin 3-mediated pyruvate dehydrogenase activity determines brown adipocytes phenotype under high-salt conditions

Fig. 6

a The mass spectrum of PDHA1 acetylation sites in SIRT3-KO mice compared with WT mice. b The expression of PDHA1 K83-Ac and c PDH activity in WT, SIRT3-KO and SIRT3-reconstruction brown adipocytes treated with or without NaCl. d Differentiated brown adipocytes stained with PDHA1 K83-Ac antibody (red); nuclei were stained with DAPI (blue). Scale bar: 50 μm. Representative images of brown adipocytes pretreated with PDHA1 K83-Ac peptide e or DCA f labelled with complex I (green) and UCP-1 (red). Scale bar: 50 μm. g Representative western blots and h semi-quantitative analyses of perilipin-1, UCP-1 and complex I in PDHA1 K83-Ac peptide or i, j DCA pretreated brown adipocytes. Results are shown as fold increase relative to the WT control and are presented as mean ± SEM of four independent experiments. *P < 0.05, **P < 0.01 vs. WT control; #P < 0.05, ##P < 0.01 vs. WT-NaCl. $P < 0.05 vs. SIRT3-KO-NaCl

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