Fig. 2: Characteristics of LINC00511 in ER-negative breast cancer.

a Scatter diagram depiction of LINC00511 expression in multiple cancer tissues and adjacent normal tissues generated from the GEPIA database. b Scatter diagram depiction of LINC00511 expression in ER-positive and ER-negative breast cancer tissues generated from the TCGA database. c Scatter diagram depiction of LINC00511 expression in ER-positive and ER-negative breast cancer cells generated from the CCLE database. d qRT-PCR analysis of LINC00511 expression following the knockdown of ESR1 expression in MCF7 and T47D cell lines. e qRT-PCR analysis of LINC00511 expression in MCF7 cells treated with different doses of tamoxifen. f The upper corner of the picture showed the TFAP-2 binding motif, and the lower table showed the prediction of TFAP-2 binding sites within the promoter region of LINC00511 provided by the JASPAR database. g Analysis of TFAP-2 ChIP-seq data from MCF7 cells at the LINC00511 promoter locus generated from data from the ENCODE and Cistrome Data Browser databases. h qRT-PCR analysis of the knockdown efficiency of TFAP-2 expression in UACC-812 and MDA-MB-231 cells. i qRT-PCR analysis of LINC00511 expression in UACC-812 and MDA-MB-231 cell lines following the knockdown of TFAP-2 expression. j Scatter diagram analysis of the correlation between TFAP-2 and LINC00511 generated from the CCLE database. k Scatter diagram analysis of the correlation between TFAP-2 and LINC00511 generated from the TCGA database. l ChIP and qRT-PCR analysis of TFAP-2 occupancy at the LINC00511 promoter region and the altered occupancy efficiency following the knockdown of ER expression in MCF7 cells. IgG was used as a negative control. Enrichment was quantified relative to input controls. Data are shown as the mean ± SD. Student’s t test was used for the statistical analysis: *p < 0.05; **p < 0.01; ***p < 0.001. Data represent three independent experiments