Fig. 3: OSI promotes autophagic flux in CRC cells. | Cell Death & Disease

Fig. 3: OSI promotes autophagic flux in CRC cells.

From: MCT1 relieves osimertinib-induced CRC suppression by promoting autophagy through the LKB1/AMPK signaling

Fig. 3

a Immunofluorescence analysis of cells transiently transfected with tandem mRFP-GFP-tagged LC3 and treated with or without 5 μM OSI in the presence or absence of 10 μM CQ for 24 h. Scale bar, 10 μm. b Quantification of the ratio of red puncta indicating autolysosome (AL) versus yellow puncta indicating autophagosome (AP) per cell in (a). c CRC cells were treated with or without 5 μM OSI in the presence or absence of 10 μM CQ for 24 h. LC3 expression was examined by immunoblotting. d Representative images of CRC cells incubated with BODIPY-conjugated bovine serum (DQ-BSA, red) for 1 h and followed by 5 μM OSI treatment for 24 h, or incubation with serum- and glucose-free medium (Starvation). Scale bar, 20 μm. e The intensity of fluorescent signal in (d). Data are presented as mean SEM, Student’s t-test, and are representative of three independent experiments. **P < 0.01; ***P < 0.001

Back to article page