Fig. 6: ERβ induction results in decrease in cellular proliferation and migration.

a Cell proliferation assay measured in MDA MB 468 cells treated with either 10 nM DPN or DMSO kept for 15 days and as observed from colony formation capacity (n = 3). The size and number of colonies are represented in the bar diagram. b Scratch assay performed on MDA MB 468 cells subjected to DPN or DMSO and at the indicated time slots for a total period of 24 hr. Scale bar of 1000 μm applies to all the images. Percentage of the gap remaining measured is represented as bar diagram. c Cell viability as determined by MTT Assay in multiple TNBC cell lines treated with increasing concentration of DPN for 48 hr. d MDA MB 468 cells transfected with siCon or siPML and treated with or without 10 nM DPN and cell viability determined by MTT assay as percentage of untransfected cells at the end of 72 hr. e Caspase3/7 activity measured in MDA MB 468 cells treated with DPN or DMSO as an increase in luminescence, either 30 min or 1.5 hr post addition of the reagent (mean of three separate experiments and ±s.e.m. calculated using Student’s t-test). f MDA MB 468 cells transfected with siCon or siPML and treated with or without DPN and Caspase3/7 activity measured 30 min post addition of the reagent (mean of three separate experiments, ±s.e.m. calculated using Student’s t-test). g Cell cycle distribution measured by flow cytometry in MDA MB 468 cells transfected with exogenous WT-ERβ and further transfected with either scrambled siRNA or with siRNA against PML. h Cell cycle distribution measured by flow cytometry in MDA MB 468 cells transfected with either scrambled siRNA or with siRNA against PML and further transfected with WT-ERβ. Error bars represent mean (±) s.d. calculated from three independent experiments. P < 0.0001 is represented as **** for highly significant and NS denotes ‘not significant’