Fig. 3: Effects of WZ811 on BLM-induced pulmonary fibrosis.

a Flow cytometry analysis of CD41⁺ cells (megakaryocyte) in the lung tissues of different groups. b Relative percentage of CD41⁺ cells (megakaryocyte) in different groups (n = 3, *P < 0.05 vs control group. ^#P < 0.05 vs BLM group). c Representative micrographs of H&E staining, Masson’s trichrome and immunohistochemical staining of lung sections for Col III and ɑ-SMA (Bar = 50 μm). d Ashcroft score was used to evaluate the degree of fibrosis (n = 5, **P < 0.01 vs control group. ^##P < 0.01 vs BLM group). e Collagen content was measured by hydroxyproline (HYP) assay (n = 4, **P < 0.01 vs control group. ^##P < 0.01 vs BLM group). f The expression of ɑ-SMA mRNA in the lung tissue of mice detected by qPCR (n = 3, *P < 0.05 vs control group. ^#P < 0.05 vs BLM group). g The content of ɑ-SMA in lung tissue detected by western blotting (n = 3, **P < 0.01 vs control group. ^##P < 0.01 vs BLM group). h The expression of TGF-β1 mRNA in the lung tissue of mice detected by qPCR (n = 3, *P < 0.05 vs control group. ^#P < 0.05 vs BLM group). iThe content of TGF-β1 protein in lung homogenate of mouse detected by ELISA (n = 3, **P < 0.01 vs control group. ^##P < 0.01 vs BLM group). The data are presented as the mean ± SD