Fig. 4: Effects of M-07e on the primary lung fibroblast.
From: Megakaryocytes participate in the occurrence of bleomycin-induced pulmonary fibrosis
(a) Left: Microscopic appearance of primary lung fibroblasts (third passages); Middle: immunofluorescence for Vimentin; right: immunofluorescence for proSP-C (Bar = 30 μm). b Experimental design: ① direct co-culture assay; ② transwell co-culture assay. M-07e in the upper chamber, fibroblasts in the lower chamber. c Growth of primary lung fibroblasts, evaluated by the CCK-8 assay (n = 6, **P < 0.01 vs control group). Fibroblasts directly co-culture with different density of M-07e. M1: 5×103/ml; M2: 104/ml; M3: 2×104/ml. d The level of ɑ-SMA evaluated by western blotting. Fibroblasts directly co-culture with different density of M-07e (n = 3, **P < 0.01 vs control group). M1: 5×103/ml; M2: 104/ml; M3: 2×104/ml. e The level of ɑ-SMA evaluated by western blotting. Fibroblasts transwell co-culture with different density of M-07e (n = 3). M1: 5×103/ml; M2: 104/ml; M3: 2×104/ml. f Immunofluorescence for Ki-67 of fibroblasts (Bar = 30 μm). Co-M-07e: 2×104/ml M-07e co-culture with fibroblast. g Data analysis of the percentage of Ki-67+ to DAPI (n = 3, *P < 0.05 vs control group). h Immunofluorescence for ɑ-SMA of fibroblasts (Bar = 30 μm). The data are presented as the mean ± SD
