Fig. 5: Effects of TPO-induced M-07e on the primary lung fibroblast.
From: Megakaryocytes participate in the occurrence of bleomycin-induced pulmonary fibrosis
a Experimental design: ③ Conditioned medium (CM) of different concentration of TPO inducing M-07e to culture fibroblasts. b Cell growth of primary lung fibroblasts, evaluated by the CCK-8 assay (n = 6). Fibroblasts culture with different concentration TPO. c The level of ɑ-SMA evaluated by western blotting. Fibroblasts culture with different concentration TPO. d Semi-quantitative analysis of ɑ-SMA level evaluated by western blotting. Fibroblasts culture with different concentration TPO (n = 6). e The level of ɑ-SMA and Col III evaluated by western blotting. Fibroblasts culture with CM of M-07e inducing with different concentration TPO. f Semi-quantitative analysis of ɑ-SMA level evaluated by western blotting (n = 3, **P < 0.01 vs control group). g Semi-quantitative analysis of Col III level evaluated by western blotting (n = 3, *P < 0.05, **P < 0.01 vs control group). h Immunofluorescence for ɑ-SMA of fibroblasts cultured with CM of M-07e inducing with 30 ng/ml TPO (Bar = 30 μm, CM-T30 group: CM of 30 ng/ml TPO stimulation to M-07e). i Immunofluorescence for Col III of fibroblasts cultured with CM of M-07e inducing with 30 ng/ml TPO (Bar = 60 μm). The data are presented as the mean ± SD
