Fig. 6: M-07e promoted the proliferation of primary lung fibroblast and trans-differentiation to myofibroblast through TGF-β1 pathway.
From: Megakaryocytes participate in the occurrence of bleomycin-induced pulmonary fibrosis
a The level of TGF-β1 of M-07e inducing with different concentration TPO evaluated by western blotting (n = 3, **P < 0.01 vs control group). b Immunofluorescence for Ki-67 of fibroblasts cultured with CM of M-07e inducing with 30 ng/ml TPO with or not with Repsox (Bar = 60 μm). c Data analysis of the percentage of Ki-67+ to DAPI (n = 3, *P < 0.05, **P < 0.01 vs control group. #P < 0.05 vs CM-T30 group). d The level of ɑ-SMA and Col III evaluated by western blotting. e Semi-quantitative analysis of ɑ-SMA level evaluated by western blotting (n = 3, *P < 0.05 vs control group. #P < 0.05 vs CM-T30 group). f Semi-quantitative analysis of Col III level evaluated by western blotting (n = 3, **P < 0.01 vs control group. ##P < 0.01 vs CM-T30 group). The data are presented as the mean ± SD
