Fig. 2: CP model established by caerulein injection in PRSS1 transgenic mice (PRSSTrans).
From: ATF6 regulates the development of chronic pancreatitis by inducing p53-mediated apoptosis
a Histological evaluation of pancreatic tissues collected from chronic pancreatitis (CP) model mice and wild-type (WT) mice by haematoxylin and eosin (H&E) staining. b Analysis of collagen deposition by Masson’s trichrome staining (Red (↑) and yellow arrows (↑) indicate increased fibrosis in pancreatic tissues from PRSS1 transgenic mice at 2 and 4 weeks post-caerulein injection in pancreatic tissues from PRSS1 transgenic mice, respectively.) and c collagen I levels and d α-smooth muscle actin (α-SMA) levels by immunohistochemistry (IHC) in pancreatic tissues from CP model mice and WT mice sacrificed 1, 2, or 4 weeks post-caerulein injection. e IHC histological scores of pancreatic tissues in CP model mice after finishing caerulein treatment. The levels of IL-1β, IL-6, and TNF-α in pancreatic tissues (f) and serum (g) from WT and PRSS1 transgenic mice treated with caerulein for CP induction, as determined by ELISA and quantitative RT-PCR. PRSS1 serine protease 1, α-SMA α-smooth muscle actin, IL-1β interleukin 1β, IL-6 interleukin 6, TNF-α tumour necrosis factor-α, BL baseline, W weeks, ns no significant difference; *P < 0.05
