Fig. 1: Loss of XIAP results in TNFR2-mediated cell death in myeloid cells. | Cell Death & Disease

Fig. 1: Loss of XIAP results in TNFR2-mediated cell death in myeloid cells.

From: TNFR2 induced priming of the inflammasome leads to a RIPK1-dependent cell death in the absence of XIAP

Fig. 1

a, b, d, f Cell death was measured by the uptake of propidium iodide (PI) and analyzed by flow cytometry. a BMDMs from wildtype (WT) and xiap−/− were stimulated with Birinapant (Biri, 500 nM) and recombinant mouse TNF (100 ng/ml) overnight. b BMDMs from WT, xiap−/−, ciap1−/− and ciap2−/− mice were treated overnight with TR1-TNF (100 ng/ml) or TNC-TNF (100 ng/ml). c Representative phase-contrast images merged with PI positive images at 0, 8 and 18 h after TNC-TNF stimulation (3 independent experiments performed). d BMDMs from WT, xiap−/− and xiapΔ−/− mice were treated overnight with TR1-TNF or TNC-TNF. e Basal expression levels of XIAP in HoxB8 progenitors were assessed by western blot. Blot is a representative of three independent experiments. f Xiap−/− HoxB8 granulocytes were transfected with WT, XIAP or XIAP mutated constructs and stimulated with TR1-TNF or TNC-TNF for 24 h. a, b, d Data shown are mean ± SEM including n = 3–5 biological replicates. Experiments were repeated at least three times independently or f a minimum of three independent experiments, including triplicates for each experiment. Statistical significance was calculated using (a, b, d) two-way ANOVA or (f) Student t-test with **p < 0.01, ***p < 0.001 and ****p < 0.0001.

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