Fig. 1: Analysis of miR-29a actions to osteogenic and osteoclastogenic differentiation. | Cell Death & Disease

Fig. 1: Analysis of miR-29a actions to osteogenic and osteoclastogenic differentiation.

From: MicroRNA-29a represses osteoclast formation and protects against osteoporosis by regulating PCAF-mediated RANKL and CXCL12

Fig. 1

Increased Runx2 and osteocalcin expression and von Kossa-stained mineralized matrix formation in miR-29a-transfected bone marrow mesenchymal cells (a); scale bar: 40 μm. Schematic drawing of bone marrow macrophages and mesenchymal cells co-incubated in transwell plates (b). Increasing miR-29a in osteoblasts decreased TRAP-stained osteoclast differentiation (scale bar: 8 μm) and pit formation (scale bar: 20 μm) of bone marrow macrophages (c). The data of in vitro model are expressed as mean ± SEM calculated from four experiments. Asterisks * indicate significant difference from the scrambled control. Sparse trabecular microstructure (scale bar: 5 mm) along with decreased BMD, BV/TV (d), and miR-29a expression in bone tissue (e) occurred in ovariectomized mice. Investigations are expressed mean ± SEM calculated from six mice. Asterisks * indicate significant difference from sham controls

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