Fig. 6: Thyroid morphology and differentiation in DKO newborn mice. | Cell Death & Disease

Fig. 6: Thyroid morphology and differentiation in DKO newborn mice.

From: Double knock-out of Hmga1 and Hipk2 genes causes perinatal death associated to respiratory distress and thyroid abnormalities in mice

Fig. 6

a, b H&E staining of WT and DKO thyroid glands of mice at P1 (× 200 magnification). c–j NKX2.1, FOXE1, PAX8, and TG on WT c, e, g, i and DKO d, f, h, j thyroid sections (× 20 magnification). One representative experiment is shown. k RNA extracted from portion of neck containing thyroid gland of WT and DKO mice at P1 was analyzed by qRT-PCR for Pax8, Tg, Tpo, and TSHr expression. n = 3 WT mice and n = 6 DKO mice were analyzed. The Actin expression level has been used for normalization. Data are mean ± SD of a representative experiment performed in triplicate. * indicates significant difference P < 0.05, ***P < 0.001 (Student’s t test)

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